Real-Time Quantitative PCR, Pathogen Detection and MIQE
- 13 September 2012
- book chapter
- Published by Springer Science and Business Media LLC
- Vol. 943, 1-16
- https://doi.org/10.1007/978-1-60327-353-4_1
Abstract
Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination of optimal sample selection, assay design and validation as well as appropriate data analysis and the “Minimal Information for the Publication of real-time PCR” (MIQE) guidelines aim to improve both the reliability of assay design as well as the transparency of reporting, essential conditions if qPCR is to remain the benchmark technology for molecular diagnosis.Keywords
This publication has 38 references indexed in Scilit:
- Aspergillus PCR: One Step Closer to StandardizationJournal of Clinical Microbiology, 2010
- Use of PCR for diagnosis of invasive aspergillosis: systematic review and meta-analysisThe Lancet Infectious Diseases, 2009
- Molecular diagnostic platforms for detectingAspergillusMedical Mycology, 2009
- Aspergillus PCR formidable challenges and progressMedical Mycology, 2009
- Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trendsNucleic Acids Research, 2007
- Automated screening using microfluidic chip‐based PCR and product detection to assess risk of BK virus‐associated nephropathy in renal transplant recipientsElectrophoresis, 2006
- Nanoliter high throughput quantitative PCRNucleic Acids Research, 2006
- Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virusBiosensors and Bioelectronics, 2006
- Quantitative real-time RT-PCR – a perspectiveJournal of Molecular Endocrinology, 2005
- Defining Opportunistic Invasive Fungal Infections in Immunocompromised Patients with Cancer and Hematopoietic Stem Cell Transplants: An International ConsensusClinical Infectious Diseases, 2002