Amphiphysin II (SH3P9; BIN1), a Member of the Amphiphysin/Rvs Family, Is Concentrated in the Cortical Cytomatrix of Axon Initial Segments and Nodes of Ranvier in Brain and around T Tubules in Skeletal Muscle
Open Access
- 16 June 1997
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 137 (6), 1355-1367
- https://doi.org/10.1083/jcb.137.6.1355
Abstract
Amphiphysin (amphiphysin I), a dominant autoantigen in paraneoplastic Stiff-man syndrome, is a neuronal protein highly concentrated in nerve terminals, where it has a putative role in endocytosis. The yeast homologue of amphiphysin, Rvs167, has pleiotropic functions, including a role in endocytosis and in actin dynamics, suggesting that amphiphysin may also be implicated in the function of the presynaptic actin cytoskeleton. We report here the characterization of a second mammalian amphiphysin gene, amphiphysin II (SH3P9; BIN1), which encodes products primarily expressed in skeletal muscle and brain, as differentially spliced isoforms. In skeletal muscle, amphiphysin II is concentrated around T tubules, while in brain it is concentrated in the cytomatrix beneath the plasmamembrane of axon initial segments and nodes of Ranvier. In both these locations, amphiphysin II is colocalized with splice variants of ankyrin3 (ankyrinG), a component of the actin cytomatrix. In the same regions, the presence of clathrin has been reported. These findings support the hypothesis that, even in mammalian cells, amphiphysin/Rvs family members have a role both in endocytosis and in actin function and suggest that distinct amphiphysin isoforms contribute to define distinct domains of the cortical cytoplasm. Since amphiphysin II (BIN1) was reported to interact with Myc, it may also be implicated in a signaling pathway linking the cortical cytoplasm to nuclear function.Keywords
This publication has 64 references indexed in Scilit:
- Synaptic Vesicle Endocytosis Impaired by Disruption of Dynamin-SH3 Domain InteractionsScience, 1997
- Identification of a small cytoplasmic ankyrin (AnkG119) in the kidney and muscle that binds beta I sigma spectrin and associates with the Golgi apparatus.The Journal of cell biology, 1996
- Characterization of a second human clathrin heavy chain polypeptide gene (CLH-22) from chromosome 22q11Human Molecular Genetics, 1996
- Chromosomal Localization of the AnkyrinG Gene (ANK3/Ank3) to Human 10q21 and Mouse 10Genomics, 1995
- The Appendage Domain of α-Adaptin Is a High Affinity Binding Site for DynaminOnline Journal of Public Health Informatics, 1995
- Immunolocalization studies of putative guidance molecules used by axons and growth cones of intersegmental interneurons in the chick embryo spinal cordJournal of Comparative Neurology, 1991
- Localization of anti-clathrin antibody in the sarcomere and sensitivity of myofibril structure to chloroquine suggest a role for clathrin in myofibril assemblyExperimental Cell Research, 1990
- Localization of the α1 and α2 subunits of the dihydropyridine receptor and ankyrin in skeletal muscle triadsNeuron, 1990
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970