YjgF Is Required for Isoleucine Biosynthesis whenSalmonella entericaIs Grown on Pyruvate Medium
- 15 April 2008
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 190 (8), 3057-3062
- https://doi.org/10.1128/jb.01700-07
Abstract
The YjgF/YER057c/UK114 family of proteins is conserved across the three domains of life, yet no biochemical function has been clearly defined for any member of this family. InSalmonella enterica, a deletion ofyjgFresults in a requirement for isoleucine when the mutant strain is grown in glucose-serine or pyruvate medium. Feedback inhibition of IlvA is required for the curative effect of isoleucine on glucose-serine medium. On pyruvate medium,yjgFmutants are unable to synthesize enough isoleucine for growth. From this study, we conclude that the isoleucine requirement of ayjgFmutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity that has previously been characterized in these mutants.Keywords
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