Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy
- 24 February 2009
- journal article
- research article
- Published by Optica Publishing Group in Optics Express
- Vol. 17 (5), 3651-3658
- https://doi.org/10.1364/oe.17.003651
Abstract
We theoretically show that the shot-noise-limited sensitivity of stimulated Raman scattering (SRS) microscopy, which enables high-contrast vibrational imaging, is similar to that of coherent anti-Stokes Raman scattering microscopy. We experimentally confirm that the sensitivity of our SRS microscope is lower than the shot-noise limit only by <15 dB, which indicates that the high-sensitivity of SRS microscopy is readily available.Keywords
This publication has 13 references indexed in Scilit:
- Two-color, two-photon, and excited-state absorption microscopyJournal of Biomedical Optics, 2007
- High-sensitivity vibrational imaging with frequency modulation coherent anti-Stokes Raman scattering (FM CARS) microscopyOptics Letters, 2006
- Stimulated parametric emission microscopyOptics Express, 2006
- Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopyProceedings of the National Academy of Sciences, 2005
- Coherent anti-Stokes Raman scattering spectral interferometry: determination of the real and imaginary components of nonlinear susceptibility χ^(3) for vibrational microscopyOptics Letters, 2004
- Polarization coherent anti-Stokes Raman scattering microscopyOptics Letters, 2001
- Vibrational Imaging with High Sensitivity via Epidetected Coherent Anti-Stokes Raman Scattering MicroscopyPhysical Review Letters, 2001
- High-Resolution Nonlinear Optical Imaging of Live Cells by Second Harmonic GenerationBiophysical Journal, 1999
- Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman ScatteringPhysical Review Letters, 1999
- Nonlinear scanning laser microscopy by third harmonic generationApplied Physics Letters, 1997