Preparation and neutralization characteristics of an anti-CSF antibody

Abstract

Nine of ten rabbits immunized with a partially purified L‐cell CSF had demonstrable titers of anti‐CSF activity. In vitro the antibody was markedly inhibitory to both post‐endotoxin mouse sera and several mouse tissue extracts. CSF containing conditioned media prepared from a number of sources showed variable inhibition suggesting that murine CSF's may be characterized by marked antigenic differences. Human sources of CSF were also inhibited thus indicating a degree of cross‐reactivity between murine and human factors. These studies may provide the initial steps toward definition of the role of CSF in vivo.