Factors Affecting the Induction of Secondary Dormancy in Lettuce

Abstract

The relationship between the temperature at which germination of 50% of the seeds is inhibited in the light (GT₅₀ Light) and secondary dormancy was investigated in three cultivars of Lactuca sativa L. Seeds were incubated for varying periods under non-germinating conditions and subsequent germination in response to red light (R) was determined over a wide range of temperatures. Dark incubation at 32 C reduced the GT₅₀ Light of cv. New York but did not affect germination at temperatures below 24 C. Dark, 32 C incubation had no effect on the GT₅₀ Light of cv. Great Lakes. In cv. Grand Rapids, dark incubation at 15, 24, 32, or 35 C initially reduced the GT₅₀ Light. However, longer incubations induced a secondary dormancy, i.e., the seeds became unable to germinate at all temperatures in response to R given after the high temperature incubation. A single exposure to R at the beginning of a 32 C incubation slowed the induction of secondary dormancy. Repeated exposures to R prevented the induction of secondary dormancy, but did not prevent a decline in the GT₅₀ Light. GA₃ mimicked the effect of repeated R. The differences in the germination behavior of the three cultivars suggest that there may be qualitative differences in the germination mechanism of these cultivars. This research demonstrates the significance of monitoring germination at a range of temperatures to avoid misinterpretation of the data.