Effects of insulin and high glucose on mobilization of slo1 BKCa channels in podocytes

Abstract

Podocytes are dynamic polarized cells that lie on the surface of glomerular capillaries and comprise an essential component of the glomerular filitration barrier. Podocytes are affected in the earliest stages of diabetic nephropathy and insulin signaling to podocytes is essential for normal glomerular function. Large‐conductance Ca²⁺‐activated K⁺ channels (BK_Ca channels) encoded by the Slo1 gene are expressed in podocytes in a complex with multiple glomerular slit diaphragm proteins including nephrin, TRPC6 channels, and several different actin‐binding proteins. Here we show that insulin increases cell surface expression of podocyte BK_Ca channels, which is accompanied by a corresponding increase in the density of current flowing through these channels. Insulin stimulation of BK_Ca channels was detectable in 15 min and required activation of both Erk and Akt signaling cascades. Exposure to high glucose (36.1 mM) for 24 h caused a marked reduction in the steady‐state surface expression of BK_Ca channels as well as of the slit diaphragm signaling molecule nephrin. High glucose treatment also abolished the stimulatory effects of insulin on BK_Ca current density, although insulin continued to increase phosphorylation of Erk and Akt under those conditions. Therefore, in contrast to most other cell types, high glucose abrogates the effects of insulin in podocytes at relatively distal steps in its signaling pathway. Insulin stimulation of BK_Ca channels in podocytes may prepare podocytes to adapt to changes in pressure gradients that occur during postprandial hyperfiltration. J. Cell. Physiol. 226: 2307–2315, 2011.