Albumin−CdTe Nanoparticle Bioconjugates: Preparation, Structure, and Interunit Energy Transfer with Antenna Effect
- 16 May 2001
- journal article
- research article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 1 (6), 281-286
- https://doi.org/10.1021/nl015519n
Abstract
Conjugates of bovine serum albumin and CdTe nanoparticles capped with l-cysteine have been synthesized via a one-pot glutaric dialdehyde cross-linking procedure. Diads (1:1) with some amount of 2:1 albumin−nanoparticle assemblies preferably form in this reaction, as evidenced by gel electrophoresis. Circular dichroism spectroscopy demonstrates that the tertiary structure of the protein remains largely intact after the conjugation. Attachment of protein moieties result in a significant increase of CdTe emission, which is attributed to the resonance energy transfer from the tryptophan moieties of albumin to CdTe nanoparticles acting as receptors for the protein antennae.Keywords
This publication has 31 references indexed in Scilit:
- Self-Assembly of CdSe−ZnS Quantum Dot Bioconjugates Using an Engineered Recombinant ProteinJournal of the American Chemical Society, 2000
- Studies of the antenna effect in polymer molecules. 28. Photo-Fries rearrangement of 1-naphthyl acetate in aqueous solutions of poly(sodium styrenesulfonate-co-2-vinylfluorene)New Journal of Chemistry, 1999
- Thionine Covalently Tethered to Multilayer Horseradish Peroxidase in a Self-Assembled Monolayer as an Electron-Transfer MediatorAnalytical Chemistry, 1998
- Quantum size colloids: From size-dependent properties of discrete particles to self-organized superstructuresCurrent Opinion in Colloid & Interface Science, 1998
- Organization of 'nanocrystal molecules' using DNANature, 1996
- A DNA-based method for rationally assembling nanoparticles into macroscopic materialsNature, 1996
- 1H and 113Cd NMR Investigations of Cd2+ and Zn2+ Binding Sites on Serum Albumin: Competition with Ca2+, Ni2+, Cu2+, and Zn2+Inorganic Chemistry, 1996
- Multichromophoric Cyclodextrins. 4. Light Conversion by Antenna EffectJournal of the American Chemical Society, 1996
- Glucose Sensitivity of Thiol-modified Gold Electrodes Having Immobilized Glucose Oxidase and 2-AminoethylferroceneChemistry Letters, 1993
- Self-assembly and photophysical properties of lanthanide dinuclear triple-helical complexesJournal of the American Chemical Society, 1993