A Method for Obtaining Longitudinal Cryostat Sections of Living Muscle Without Contraction Artifacts
- 1 January 1974
- journal article
- Published by Taylor & Francis Ltd in Stain Technology
- Vol. 49 (3), 143-146
- https://doi.org/10.3109/10520297409116965
Abstract
Longitudinal cryostat sections of skeletal muscle picked up with glass slides onto which a film of ethylenediaminetetracetic acid (EDTA) has been dried after dipping in a 3% EDTA solution do not show the severe retraction artifacts obtained when plain glass slides are used. Histochemical techniques work well on the longitudinal sections after the EDTA has been rinsed away and remaining traces saturated with calcium, Morphologic preservation is good. Regularly distributed histochemical reaction products are found within any one fiber.Keywords
This publication has 2 references indexed in Scilit:
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- THE SPECIFICITY OF THE HISTOCHEMICAL METHOD FOR ADENOSINE TRIPHOSPHATASJournal of Histochemistry & Cytochemistry, 1955