Identification of a Diagnostic Marker To Detect Freshwater Cyanophages of Filamentous Cyanobacteria
- 1 September 2006
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 72 (9), 5713-5719
- https://doi.org/10.1128/aem.00270-06
Abstract
Cyanophages are viruses that infect the cyanobacteria, globally important photosynthetic microorganisms. Cyanophages are considered significant components of microbial communities, playing major roles in influencing host community diversity and primary productivity, terminating cyanobacterial water blooms, and influencing biogeochemical cycles. Cyanophages are ubiquitous in both marine and freshwater systems; however, the majority of molecular research has been biased toward the study of marine cyanophages. In this study, a diagnostic probe was developed to detect freshwater cyanophages in natural waters. Oligonucleotide PCR-based primers were designed to specifically amplify the major capsid protein gene from previously characterized freshwater cyanomyoviruses that are infectious to the filamentous, nitrogen-fixing cyanobacterial genera Anabaena and Nostoc . The primers were also successful in yielding PCR products from mixed virus communities concentrated from water samples collected from freshwater lakes in the United Kingdom. The probes are thought to provide a useful tool for the investigation of cyanophage diversity in freshwater environments.Keywords
This publication has 41 references indexed in Scilit:
- Identification of Cyanophage Ma-LBP and Infection of the Cyanobacterium Microcystis aeruginosa from an Australian Subtropical Lake by the VirusApplied and Environmental Microbiology, 2005
- Nearly Identical Bacteriophage Structural Gene Sequences Are Widely Distributed in both Marine and Freshwater EnvironmentsApplied and Environmental Microbiology, 2005
- Temporal distribution of viruses, bacteria and phytoplankton throughout the water column in a freshwater hypereutrophic lakeAquatic Microbial Ecology, 2005
- Genetic diversity and population dynamics of cyanophage communities in the Chesapeake BayAquatic Microbial Ecology, 2004
- Dynamics of cyanophage-like particles and algicidal bacteria causing Microcystis aeruginosa mortalityLimnology, 2001
- Phylogeny of the Major Head and Tail Genes of the Wide-Ranging T4-Type BacteriophagesJournal of Bacteriology, 2001
- Virus-like particles associated with Lyngbya majuscula (Cyanophyta; Oscillatoriacea) bloom decline in Moreton Bay, AustraliaAquatic Microbial Ecology, 2001
- Generic Assignments, Strain Histories and Properties of Pure Cultures of CyanobacteriaMicrobiology, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Algal Virus: IsolationScience, 1963