DNA methylation in embryonic stem cells
- 6 November 2009
- journal article
- review article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 109 (1), 1-6
- https://doi.org/10.1002/jcb.22374
Abstract
Embryonic stem cells (ESCs) are pluripotent, self‐renewing cells. These cells can be used in applications such as cell therapy, drug development, disease modeling, and the study of cellular differentiation. Investigating the interplay of epigenetics, genetics, and gene expression in control of pluripotence and differentiation could give important insights on how these cells function. One of the best known epigenetic factors is DNA methylation, which is a major mechanism for regulation of gene expression. This phenomenon is mostly seen in imprinted genes and X‐chromosome inactivation where DNA methylation of promoter regions leads to repression of gene expression. Differential DNA methylation of pluripotence‐associated genes such as Nanog and Oct4/Pou5f1 has been observed between pluripotent and differentiated cells. It is clear that tight regulation of DNA methylation is necessary for normal development. As more associations between aberrant DNA methylation and disease are reported, the demand for high‐throughput approaches for DNA methylation analysis has increased. In this article, we highlight these methods and discuss recent DNA methylation studies on ESCs. J. Cell. Biochem. 109: 1–6, 2010.Keywords
This publication has 43 references indexed in Scilit:
- Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogrammingNature Biotechnology, 2009
- MicroRNAs: Target Recognition and Regulatory FunctionsCell, 2009
- Highly Integrated Single-Base Resolution Maps of the Epigenome in ArabidopsisCell, 2008
- Promoter CpG Methylation Contributes to ES Cell Gene Regulation in Parallel with Oct4/Nanog, PcG Complex, and Histone H3 K4/K27 TrimethylationCell Stem Cell, 2008
- Generation of germline-competent induced pluripotent stem cellsNature, 2007
- Chromosome-wide and promoter-specific analyses identify sites of differential DNA methylation in normal and transformed human cellsNature Genetics, 2005
- Aberrant CpG-island methylation has non-random and tumour-type–specific patternsNature Genetics, 2000
- The language of covalent histone modificationsNature, 2000
- Role for DNA methylation in genomic imprintingNature, 1993
- CpG Islands in vertebrate genomesJournal of Molecular Biology, 1987