Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo
- 1 March 1984
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 308 (5956), 241-246
- https://doi.org/10.1038/308241a0
Abstract
To determine whether sequence context influences the ability of an AUG triplet to be recognized as an initiator codon by eukaryotic ribosomes, single nucleotide changes were introduced near the translational start site in a cloned preproinsulin gene. Maximum synthesis of preproinsulin occurred when a purine, preferably adenosine, was located 3 nucleotides upstream from the initiator codon. Adenosine is found most frequently in that position in natural mRNA.This publication has 25 references indexed in Scilit:
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