Nonviral Gene Delivery to Mesenchymal Stem Cells Using Cationic Liposomes for Gene and Cell Therapy
Open Access
- 24 June 2010
- journal article
- research article
- Published by Hindawi Limited in Journal of Biomedicine and Biotechnology
- Vol. 2010, 1-12
- https://doi.org/10.1155/2010/735349
Abstract
Mesenchymal stem cells (MSCs) hold a great promise for application in several therapies due to their unique biological characteristics. In order to harness their full potential in cell-or gene-based therapies it might be advantageous to enhance some of their features through gene delivery strategies. Accordingly, we are interested in developing an efficient and safe methodology to genetically engineer human bone marrow MSC (BM MSC), enhancing their therapeutic efficacy in Regenerative Medicine. The plasmid DNA delivery was optimized using a cationic liposome-based reagent. Transfection efficiencies ranged from ~2% to ~35%, resulting from using a Lipid/DNA ratio of 1.25 with a transgene expression of 7 days. Importantly, the number of plasmid copies in different cell passages was quantified for the first time and ~20,000 plasmid copies/cell were obtained independently of cell passage. As transfected MSC have shown high viabilities (90%) and recoveries (52%) while maintaining their multipotency, this might be an advantageous transfection strategy when the goal is to express a therapeutic gene in a safe and transient way.
Keywords
This publication has 37 references indexed in Scilit:
- Cationic liposome–DNA complexes as gene delivery vectors: Development and behaviour towards bone-like cellsActa Biomaterialia, 2009
- Quantification of plasmid DNA copies in the nucleus after lipoplex and polyplex transfectionJournal of Controlled Release, 2009
- Gene delivery to human adult and embryonic cell-derived stem cells using biodegradable nanoparticulate polymeric vectorsGene Therapy, 2009
- Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cellsBMC Cell Biology, 2008
- Concise Review: Adult Multipotent Stromal Cells and Cancer: Risk or Benefit?The International Journal of Cell Cloning, 2008
- Genetic modification of cells for transplantationAdvanced Drug Delivery Reviews, 2008
- Genetic modification of stem cells for transplantationAdvanced Drug Delivery Reviews, 2008
- Stem cell differentiation and expansion for clinical applications of tissue engineeringJournal of Cellular and Molecular Medicine, 2007
- Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statementCytotherapy, 2006
- Metabolic load and heterologous gene expressionBiotechnology Advances, 1995