Two protons are pumped from the mitochondrial matrix per electron transferred between NADH and ubiquinone
- 24 April 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 169 (2), 300-304
- https://doi.org/10.1016/0014-5793(84)80338-5
Abstract
The spectrophotometric indicators neutral red and safranine were used to determine the relative H+/e− ratios of proton uptake from the mitochondrial matrix, and the q+/e− ratios of electrical charge translocation, during oxidation of β-OH-butyrate and succinate by ferricyanide in rat liver mitochondria. With β-OH-butyrate both ratios were higher than with succinate by a factor close to 3.0. Since there is full agreement that H+/e− of proton uptake and q+/e− of charge translocation are both equal to unity for oxidation of succinate (or ubiquinol) by ferricytochrome c, the corresponding ratios for oxidation of NADH by ubiquinone and cytochrome c are near 2.0 and 3.0, respectively.Keywords
This publication has 15 references indexed in Scilit:
- Localized or delocalized protons in photophosphorylation? On the accessibility of the thylakoid lumen for ions and buffersBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1983
- Proton-Translocating Cytochrome ComplexesAnnual Review of Biochemistry, 1981
- Proton-pumping cytochrome c oxidaseBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1979
- The buffering capacity of the internal phase of thylakoids and the magnitude of the pH changes inside under flashing lightBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1979
- [69] Measurement of →H+/O in mitochondria and submitochondrial vesiclesMethods in enzymology, 1979
- Proton pump coupled to cytochrome c oxidase in mitochondriaNature, 1977
- Safranine as a probe of the mitochondrial membrane potentialFEBS Letters, 1976
- Neutral red response as a measure of the pH gradient across chloroplast membranes in the lightFEBS Letters, 1976
- A spectral shift in cytochrome a induced by calcium ionsBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1975
- The Problem of Cation‐Binding Sites in the Energized Membrane of Intact MitochondriaEuropean Journal of Biochemistry, 1973