Application of Zebrafish Model in the Suppression of Drug-Induced Cardiac Hypertrophy by Traditional Indian Medicine Yogendra Ras
Open Access
- 13 April 2020
- journal article
- research article
- Published by MDPI AG in Biomolecules
- Vol. 10 (4), 600
- https://doi.org/10.3390/biom10040600
Abstract
Zebrafish is an elegant vertebrate employed to model the pathological etiologies of human maladies such as cardiac diseases. Persistent physiological stresses can induce abnormalities in heart functions such as cardiac hypertrophy (CH), which can lead to morbidity and mortality. In the present study, using zebrafish as a study model, efficacy of the traditional Indian Ayurveda medicine “Yogendra Ras” (YDR) was validated in ameliorating drug-induced cardiac hypertrophy. YDR was prepared using traditionally described methods and composed of nano- and micron-sized metal particles. Elemental composition analysis of YDR showed the presence of mainly Au, Sn, and Hg. Cardiac hypertrophy was induced in the zebrafish following a pretreatment with erythromycin (ERY), and the onset and reconciliation of disease by YDR were determined using a treadmill electrocardiogram, heart anatomy analysis, C-reactive protein release, and platelet aggregation time-analysis. YDR treatment of CH-induced zebrafish showed comparable results with the Standard-of-care drug, verapamil, tested in parallel. Under in-vitro conditions, treatment of isoproterenol (ISP)-stimulated murine cardiomyocytes (H9C2) with YDR resulted in the suppression of drug-stimulated biomarkers of oxidative stress: COX-2, NOX-2, NOX-4, ANF, troponin-I, -T, and cardiolipin. Taken together, zebrafish showed a strong disposition as a model for studying the efficacy of Ayurvedic medicines towards drug-induced cardiopathies. YDR provided strong evidence for its capability in modulating drug-induced CH through the restoration of redox homeostasis and exhibited potential as a viable complementary therapy.This publication has 43 references indexed in Scilit:
- Optimisation of Embryonic and Larval ECG Measurement in Zebrafish for Quantifying the Effect of QT Prolonging DrugsPLOS ONE, 2013
- Improving Bioscience Research Reporting: The ARRIVE Guidelines for Reporting Animal ResearchPLoS Biology, 2010
- A Comparative Study of Embedded and Anesthetized Zebrafish in vivo on Myocardiac Calcium Oscillation and Heart Muscle ContractionFrontiers in Pharmacology, 2010
- Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissueNature Protocols, 2009
- Mercury in Traditional Medicines: Is Cinnabar Toxicologically Similar to Common Mercurials?Experimental Biology and Medicine, 2008
- Regulation of cardiac hypertrophy by intracellular signalling pathwaysNature Reviews Molecular Cell Biology, 2006
- A Quantitative Nitroblue Tetrazolium Assay for Determining Intracellular Superoxide Anion Production in Phagocytic CellsJournal of Immunoassay and Immunochemistry, 2006
- Does load-induced ventricular hypertrophy progress to systolic heart failure?American Journal of Physiology-Heart and Circulatory Physiology, 2005
- Association of platelet function in hypertensive patients with left ventricular hypertrophy, transient myocardial ischemia, and coronary artery diseasePlatelets, 1998
- Role of cardiac angiotensin II in isoproterenol-induced left ventricular hypertrophy.Hypertension, 1992