Field of view advantage of conjugate adaptive optics in microscopy applications
- 10 April 2015
- journal article
- Published by Optica Publishing Group in Applied Optics
- Vol. 54 (11), 3498-3506
- https://doi.org/10.1364/ao.54.003498
Abstract
The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases the DM is placed conjugate to the microscope pupil, called pupil adaptive optics (AO). When the aberrations are spatially variant an alternative configuration involves placing the DM conjugate to the main source of aberrations, called conjugate AO. We provide a theoretical and experimental comparison of both configurations for the simplified case where spatially variant aberrations are produced by a well-defined phase screen. We pay particular attention to the resulting correction field of view (FOV). Conjugate AO is found to provide a significant FOV advantage. While this result is well known in the astronomical community, our goal here is to recast it specifically for the optical microscopy community.Keywords
Funding Information
- Aramco Services Company (6500012337)
- National Cancer Institute (NCI) (R01CA182939)
- National Science Foundation Industry/University Cooperative Research Center for Biophotonic Sensors and Systems (IIP-1065070)
- National Institutes of Health (NIH)
This publication has 27 references indexed in Scilit:
- Adaptive optical microscopy: the ongoing quest for a perfect imageLight: Science & Applications, 2014
- Modelling of multi-conjugate adaptive optics for spatially variant aberrations in microscopyJournal of Optics, 2013
- Dual-conjugate adaptive optics for wide-field high-resolution retinal imagingOptics Express, 2009
- Modelling the application of adaptive optics to wide‐field microscope live imagingJournal of Microscopy, 2007
- Laboratory MCAO Test-Bed for Developing Wavefront Sensing ConceptsOptics Express, 2005
- Isoplanatism in a multiconjugate adaptive optics systemJournal of the Optical Society of America A, 2000
- Adaptive-optics corrections available for the whole skyNature, 2000
- Analysis of multiconjugate adaptive opticsJournal of the Optical Society of America A, 1994
- Anisoplanatism in adaptive opticsJournal of the Optical Society of America, 1982
- V The Effects of Atmospheric Turbulence in Optical AstronomyPublished by Elsevier BV ,1981