Study Design Controlled, cadaveric implantation trial. Objective To evaluate the effect of a robotic guidance system on screw placement accuracy, amount of radiation exposure, and length of procedure time during percutaneous pedicle screw implantation. Summary of Background Data Pedicle screws are associated with low complication rates, and several computer-assisted image guidance systems exist that facilitate accurate screw placement. However, these systems may represent substantial radiation exposure risk to patients and surgeons. Methods We implanted 234 pedicle screws in 12 cadavers (study group: 15 surgeons, 197 screws, and 10 specimens; control group: 2 surgeons, 37 screws, and 2 specimens). We measured procedure time, fluoroscopy time, and radiation exposure and evaluated screw placement accuracy with computed tomography scans. To evaluate the learning curve, we compared measurements with those of an experienced robotic guidance user through the 2-sample (heteroscedastic), 1-tail t test (P< 0.05). Results Relative to control, the study group had fewer screw placement deviations (average, 2.6±0.7 mm vs. 1.1±0.4 mm; P4 mm), new-user radiation exposure (by 98.2%), and procedure time (by 36%). Conclusions The advantages associated with a robotic guidance system may make the surgeon more at ease about offering minimally invasive or percutaneous surgical options to patients and more comfortable about implementing pedicle-based fixation in general. This advanced technology may also allow inclusion of patients with complicated anatomic deformities, who are often excluded from pedicle screw-based surgery options.