SARS-like WIV1-CoV poised for human emergence
- 14 March 2016
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 113 (11), 3048-3053
- https://doi.org/10.1073/pnas.1517719113
Abstract
Outbreaks from zoonotic sources represent a threat to both human disease as well as the global economy. Despite a wealth of metagenomics studies, methods to leverage these datasets to identify future threats are underdeveloped. In this study, we describe an approach that combines existing metagenomics data with reverse genetics to engineer reagents to evaluate emergence and pathogenic potential of circulating zoonotic viruses. Focusing on the severe acute respiratory syndrome (SARS)-like viruses, the results indicate that the WIV1-coronavirus (CoV) cluster has the ability to directly infect and may undergo limited transmission in human populations. However, in vivo attenuation suggests additional adaptation is required for epidemic disease. Importantly, available SARS monoclonal antibodies offered success in limiting viral infection absent from available vaccine approaches. Together, the data highlight the utility of a platform to identify and prioritize prepandemic strains harbored in animal reservoirs and document the threat posed by WIV1-CoV for emergence in human populations.Keywords
Funding Information
- HHS | NIH | National Institute of Allergy and Infectious Diseases (U19AI109761)
- HHS | NIH | National Institute of Allergy and Infectious Diseases (U19AI107810)
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases (AI1085524)
- HHS | NIH | National Institute of Allergy and Infectious Diseases (F32AI102561)
- HHS | NIH | National Institute on Aging (K99AG049092)
- HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (DK065988)
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