Biochemical Engineering Approaches for Increasing Viability and Functionality of Probiotic Bacteria
Open Access
- 2 June 2016
- journal article
- review article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 17 (6), 867
- https://doi.org/10.3390/ijms17060867
Abstract
The literature presents a growing body of evidence demonstrating the positive effect of probiotics on health. Probiotic consumption levels are rising quickly in the world despite the fluctuation of their viability and functionality. Technological methods aiming at improving probiotic characteristics are thus highly wanted. However, microbial metabolic engineering toolbox is not available for this kind of application. On the other hand, basic microbiology teaches us that bacteria are able to exhibit adaptation to external stresses. It is known that adequately applied sub-lethal stress, i.e., controlled in amplitude and frequency at a given stage of the culture, is able to enhance microbial robustness. This property could be potentially used to improve the viability of probiotic bacteria, but some technical challenges still need to be overcome before any industrial implementation. This review paper investigates the different technical tools that can be used in order to define the proper condition for improving viability of probiotic bacteria and their implementation at the industrial scale. Based on the example of Bifidobacterium bifidum, potentialities for simultaneously improving viability, but also functionality of probiotics will be described.This publication has 127 references indexed in Scilit:
- Adaptive laboratory evolution – principles and applications for biotechnologyMicrobial Cell Factories, 2013
- Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protectionProceedings of the National Academy of Sciences of the United States of America, 2012
- Life, Death, and In-Between: Meanings and Methods in MicrobiologyApplied and Environmental Microbiology, 2011
- Potentials of Exopolysaccharides from Lactic Acid BacteriaIndian Journal of Microbiology, 2011
- The Bps polysaccharide of Bordetella pertussis promotes colonization and biofilm formation in the nose by functioning as an adhesinMolecular Microbiology, 2010
- Effect of CO 2 on Colony Development by Bifidobacterium SpeciesApplied and Environmental Microbiology, 2007
- The EPS Matrix: The “House of Biofilm Cells”Journal of Bacteriology, 2007
- Low-pH Adaptation and the Acid Tolerance Response of Bifidobacterium longum Biotype longumApplied and Environmental Microbiology, 2007
- Aspartate Biosynthesis Is Essential for the Growth of Streptococcus thermophilus in Milk, and Aspartate Availability Modulates the Level of Urease ActivityApplied and Environmental Microbiology, 2007
- Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilusNature Biotechnology, 2004