Phorbol diester-induced macrophage differentiation of leukemic blasts from patients with human myelogenous leukemia.
Open Access
- 1 November 1980
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 66 (5), 1101-1108
- https://doi.org/10.1172/jci109939
Abstract
Phorbol esters, including 12-O-tetradecanoylphorbol 13-acetate (TPA), induce terminal macrophagelike differentiation of cells from human acute myelogenous leukemia lines. We report that myelogenous leukemia cells obtained from patients undergo macrophagelike differentiation after exposure to TPA. The myeloid leukemic cell cultured with TPA became adherent to charged surfaces with long filamentous pseudopodia; developed positive staining for alpha-napthyl acetate esterase, increased lysozyme secretion, reduced nitroblue tetrazolium, and acquired the ability to phagocytose candida. Cells from patients with lymphocytic leukemia did not become macrophagelike when cultured with TPA.This publication has 38 references indexed in Scilit:
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