In situactivation of bis-dialkylaminophosphines—a new method for synthesizing deoxyoligonucleotides on polymer supports

Abstract

Deoxynucleoside phosphoramidites can be prepared in good yield from deoxynucleosides, bis-dialkylaminophosphines, and the corresponding dialkylamine hydrotetrazolide or tetrazole as catalysts. These phosphoramidites generated in situ lead to the direct synthesis of deoxyoligonucleotides on polymer supports.