A pathway‐specific transcriptional regulatory gene for nikkomycin biosynthesis in Streptomyces ansochromogenes that also influences colony development
Open Access
- 26 January 2005
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 55 (6), 1855-1866
- https://doi.org/10.1111/j.1365-2958.2005.04512.x
Abstract
DNA sequence analysis of a 7.5 kb XhoI DNA fragment from the region flanking the nikkomycin biosynthesis gene cluster in Streptomyces ansochromogenes revealed one 3.3 kb open reading frame (ORF), designated sanG. The deduced product of sanG (1061 amino acids), which is similar to PimR of Streptomyces natalensis, contains an OmpR-like DNA binding domain in its N-terminal portion and A- and B-type nucleotide binding motifs in the middle of the protein. Disruption of sanG abolished nikkomycin biosynthesis, reduced sporulation and led to brown pigment accumulation. All aspects of this complex phenotype were complemented by a single copy sanG which was integrated into the chromosome. The introduction of multiple copies of sanG resulted in increased nikkomycin production. S1 mapping results indicated that sanG is transcribed from at least three promoters (P1, P2 and P3), P1 being strongly upregulated when production of nikkomycins starts. Two putative transcription units for nikkomycin biosynthesis, starting from sanN and sanO, are dependent on the expression of sanG, whereas a putative transcription unit starting from sanF was not regulated by sanG. These results suggested that sanG encodes a transcriptional activator important for nikkomycin biosynthesis that, unusually, also has pleiotropic effects on secondary metabolism and development.Keywords
This publication has 47 references indexed in Scilit:
- Identification of PimR as a Positive Regulator of Pimaricin Biosynthesis in Streptomyces natalensisJournal of Bacteriology, 2004
- Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilisNature Biotechnology, 2003
- Pleiotropic Functions of a Streptomyces pristinaespiralis Autoregulator Receptor in Development, Antibiotic Biosynthesis, and Expression of a Superoxide DismutaseOnline Journal of Public Health Informatics, 2001
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Structural relationships in the OmpR family of winged-helix transcription factorsJournal of Molecular Biology, 1997
- Analysis of the integration function of the streptomycete bacteriophage φC31Journal of Molecular Biology, 1991
- The use of a rare codon specifically during development?Molecular Microbiology, 1991
- The act cluster contains regulatory and antibiotic export genes, direct targets for translational control by the bldA tRNA gene of streptomycesCell, 1991
- Nikkomycins: Microbial inhibitors of chitin synthaseJournal of Chemical Technology and Biotechnology, 1982
- The Polyoxins: Pyrimidine Nucleoside Peptide Antibiotics Inhibiting Fungal Cell Wall BiosynthesisHETEROCYCLES, 1979