Ca2+INFLUX INDUCED BY PHOTODYNAMIC ACTION IN HUMAN CEREBRAL GLIOMA (U-87 MG) CELLS: POSSIBLE INVOLVEMENT OF A CALCIUM CHANNEL
- 1 September 1994
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 60 (3), 246-248
- https://doi.org/10.1111/j.1751-1097.1994.tb05098.x
Abstract
The plasma membrane has been implicated as a critical target of photodynamic action on cells. We have observed that the photosensitization of human cerebral glioma (U-87 MG) cells by hematoporphyrin derivative (HpD) causes a large increase in intracellular calcium [Ca2+]i. This increase in [Ca2+]i was solely due to the influx of extracellular Ca2+ through the plasma membrane and showed a dependence on HpD concentration, light dose and concentration of calcium in the extracellular medium. The magnitude of the Ca2+ influx decreased with increasing postirradiation time, which suggests that the cell membrane partially recovers from the photodynamic injury. The photoinduced Ca2+ influx was inhibited by the Ca2+ channel blocker diltiazem and the reducing agent dithioerythritol. These findings are discussed in terms of possible activation of a Ca2+ channel as a result of photosensitization.This publication has 24 references indexed in Scilit:
- HOW DOES PHOTODYNAMIC THERAPY WORK?Photochemistry and Photobiology, 1992
- Early increase in intracellular calcium during photodynamic permeabilizationFree Radical Biology & Medicine, 1991
- Plasma membrane depolarization and calcium influx during cell injury by photodynamic actionBiochimica et Biophysica Acta (BBA) - Biomembranes, 1991
- PTHALOCYANINE-INDUCED PHOTODYNAMIC CHANGES OF CYTOPLASMIC FREE CALCIUM IN CHINESE HAMSTER CELLSPhotochemistry and Photobiology, 1991
- MEMBRANE PHOTOMODIFICATION OF CARDIAC MYOCYTES: POTASSIUM AND LEAKAGE CURRENTSPhotochemistry and Photobiology, 1991
- DEPOLARIZATION OF MOUSE MYELOMA CELL MEMBRANES DURING PHOTODYNAMIC ACTIONPhotochemistry and Photobiology, 1990
- Enhancement of transbilayer mobility of a membrane lipid probe accompanies formation of membrane leaks during photodynamic treatment of erythrocytesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1989
- PORPHYRIN PHOTOSENSITIZATION AND PHOTOTHERAPYPhotochemistry and Photobiology, 1986
- Internal and external application of photodynamic sensitizers on squid giant axonsThe Journal of Membrane Biology, 1977
- Photodynamic alteration of lobster giant axons in calcium-free and calcium-rich mediaThe Journal of Membrane Biology, 1973