Quantitation of glucocorticoid receptor alpha and NF-κB pathway mRNA and its correlation with disease activity in rheumatoid arthritis patients
- 1 January 2010
- journal article
- Published by Genetics and Molecular Research in Evolution
- Vol. 9 (4), 2300-2310
- https://doi.org/10.4238/vol9-4gmr970
Abstract
We measured NF-κB, IKK, c-Fos, and GRα mRNA expression and in vivo glucocorticoid sensitivity in patients with rheumatoid arthritis. A very low dose intravenous dexamethasone suppression test and real-time PCR quantitation of mRNA of these genes were performed on blood samples from 21 rheumatoid arthritis patients who were not on glucocorticoids during the previous four months and on blood samples from 20 healthy individuals. Mean rheumatoid arthritis duration was 8.8 years, and mean disease activity, as assessed by Disease Activity Score 28 (DAS28), was 4.45. Basal cortisol and the percentage of cortisol reduction after the very low dose intravenous dexamethasone suppression test, as well as NF-κB, IKK, c-Fos, and GRα mRNA expression, were similar among groups. We did not observe significant correlations between glucocorticoid in vivo sensitivity and DAS28. There was a positive correlation between DAS28 and NF-κB, IKK, and GRα, but not c-Fos. In the multivariate analysis, only NF-κB mRNA remained as an independent variable for predicting DAS28.Keywords
This publication has 20 references indexed in Scilit:
- Simultaneous evaluation of in vivo glucocorticoid sensitivity and expression of glucocorticoid receptor alpha-isoform in rheumatoid arthritis patientsArchives of Endocrinology and Metabolism, 2009
- Artrite reumatóide e ateroscleroseRevista da Associação Médica Brasileira, 2007
- Guidelines for the management of rheumatoid arthritis: 2002 UpdateArthritis & Rheumatism, 2002
- Correlation of IL-6 with the classical humoral disease activity parameters ESR and CRP and with serum cortisol, reflecting the activity of the HPA axis in active rheumatoid arthritis.Zeitschrift für Rheumatologie, 2000
- Measures of activity and damage in rheumatoid arthritis: Depiction of changes and prediction of mortality over five yearsArthritis & Rheumatism, 1997
- Direct evidence of high DNA binding activity of transcription factor AP‐1 in rheumatoid arthritis synoviumArthritis & Rheumatism, 1997
- Circadian Relationships between Interleukin (IL)-6 and Hypothalamic-Pituitary-Adrenal Axis Hormones: Failure of IL-6 to Cause Sustained Hypercortisolism in Patients with Early Untreated Rheumatoid ArthritisJournal of Clinical Endocrinology & Metabolism, 1997
- NF-κB: Ten Years AfterCell, 1996
- THE NF-κB AND IκB PROTEINS: New Discoveries and InsightsAnnual Review of Immunology, 1996
- Assessment of chronic pain. I. Aspects of the reliability and validity of the visual analogue scalePain, 1983