Monoclonal Antibodies against Adenovirus Type 5: Preparation and Preliminary Characterization

Abstract

Eight different [murine] hybridoma cell lines producing monoclonal antibodies against the major antigens of human adenovirus type 5 were obtained. They were selected by screening initial hybridomas by the fluorescent antibody technique followed by radioimmune precipitation; they reacted with hexon, penton, fiber and the 100 kilodalton polypeptides. Five apparently different epitopes against the hexon antigen were detected showing a spectrum of activity against the hexons of other serotypes, suggesting that the hexon contained a variety of subgroup specificities and the previously described group and type specificities.