Two rapid and simple methods used for the removal of resins from 1.0 μm thick epoxy sections

Abstract

Ultrathin epoxy sections are commonly used in EM. More useful information can often be obtained by examining thicker (0.5-3.0 .mu.m) resin sections under the light microscope, but there are some histological stains that are unable to penetrate these resins. The easy and rapid preparation of 2 resin-removal solutions which allow subsequent staining of 1.0 .mu.m thick sections without obvious tissue damage and with excellent results using standard histological dyes is described.