K-ras,p53, andDPC4(MAD4) Alterations in Fine-Needle Aspirates of the Pancreas
Open Access
- 1 May 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 117 (5), 755-765
- https://doi.org/10.1309/5rq0-jcqu-5xf2-51lq
Abstract
Between January 1997 and February 2000, 101 fine-needle pancreatic aspirates were obtained. After a cytologic diagnosis was made, possible molecular alterations were determined on the 94 aspirates with adequate tissue using a molecular panel (K-ras, p53, and DPC4 [MAD4] genes). The 94 aspirates were categorized as follows: diagnostic of adenocarcinoma, 48 (51%); atypical (suggestive of, but not diagnostic of, adenocarcinoma), 19 (20%); negative for adenocarcinoma, 25 (27%); diagnostic of a neoplasm other than adenocarcinoma, 2 (2%). Clinical follow-up revealed that 3 patients (12%) with negative cytologic diagnoses and 12 patients (63%) with atypical cytologic diagnoses had adenocarcinoma. Of 63 with a final diagnosis of adenocarcinoma, 42 (67%) had an alteration in at least 1 of the genes analyzed. In contrast, only 2 (6%) of 31 patients without adenocarcinoma had an alteration in 1 gene on the panel. Overall, the molecular analyses supported the diagnosis of adenocarcinoma in 6 (32%) of 19 aspirates originally diagnosed as atypical by cytology alone. A molecular panel that includes the K-ras, p53, and DPC4 (MAD4) genes correlates with and can supplement traditional cytologic diagnosis of pancreatic fine-needle aspirates.Keywords
This publication has 29 references indexed in Scilit:
- Analysis of K‐ras oncogene mutation directly applied to atypical cell clusters on cytologic smear slides of bile and pancreatic juicePathology International, 1998
- Clinical significance of K-ras oncogene activation in ampullary neoplasms.Journal of Clinical Pathology, 1996
- DPC4 , A Candidate Tumor Suppressor Gene at Human Chromosome 18q21.1Science, 1996
- Prognostic Value of Immunohistochemical Expression of p53 in Patients with Pancreatic CancerOncology, 1996
- Detection of P53 protein in cytological specimens of pancreatic diseases obtained by selective endoscopic brushingGastroenterology, 1995
- Molecular markers for diagnostic cytology of neoplasms in the head region of the pancreas: mutation of K-ras and overexpression of the p53 protein product.Journal of Clinical Pathology, 1995
- Detection of Point Mutations in the Kirsten-ras Oncogene Provides Evidence for the Multicentricity of Pancreatic CarcinomaAnnals of Surgery, 1993
- A Rapid and Simple Procedure for the Routine Detection of ras Point Mutations in Formalin-Fixed, Paraffin-Embedded TissuesDiagnostic Molecular Pathology, 1992
- Immunohistochemical analysis of p53 and HER-2/neu proteins in human tumors.Journal of Histochemistry & Cytochemistry, 1991
- Frequency and Types of Point Mutation at the 12th Codon of the c‐Ki‐ras Gene Found in Pancreatic Cancers from Japanese PatientsJapanese Journal of Cancer Research, 1989