Engineering the Provitamin A (β-Carotene) Biosynthetic Pathway into (Carotenoid-Free) Rice Endosperm
Top Cited Papers
- 14 January 2000
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 287 (5451), 303-305
- https://doi.org/10.1126/science.287.5451.303
Abstract
Rice ( Oryza sativa ), a major staple food, is usually milled to remove the oil-rich aleurone layer that turns rancid upon storage, especially in tropical areas. The remaining edible part of rice grains, the endosperm, lacks several essential nutrients, such as provitamin A. Thus, predominant rice consumption promotes vitamin A deficiency, a serious public health problem in at least 26 countries, including highly populated areas of Asia, Africa, and Latin America. Recombinant DNA technology was used to improve its nutritional value in this respect. A combination of transgenes enabled biosynthesis of provitamin A in the endosperm.Keywords
This publication has 12 references indexed in Scilit:
- Plasmolysis of precultured immature embryos improves Agrobacterium mediated gene transfer to rice (Oryza sativa L.)Plant Science, 1997
- A One Year Study of the Macular Pigment: The Effect of 140 Days of a Lutein SupplementExperimental Eye Research, 1997
- Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesisThe Plant Journal, 1997
- Transgenic Indica Rice Breeding Line IR58 Expressing a Synthetic crylA(b) Gene from Bacillus thuringiensis Provides Effective Insect Pest ControlNature Biotechnology, 1996
- The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformationPlant Molecular Biology, 1994
- Functional expression of the Erwinia uredovora carotenoid biosynthesis gene crtl in transgenic plants showing an increase of β‐carotene biosynthesis activity and resistance to the bleaching herbicide norflurazonThe Plant Journal, 1993
- Vitamin A and Infection: Public Health ImplicationsAnnual Review of Nutrition, 1989
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmidNature, 1983
- Vitamin A deficiency and child blindness in the developing worldProceedings of the Nutrition Society, 1983