A Single Course of Prenatal Betamethasone in the Rat Alters Postnatal Brain Cell Proliferation but not Apoptosis
- 1 October 2003
- journal article
- Published by Wiley in Journal Of Physiology-London
- Vol. 552 (1), 163-175
- https://doi.org/10.1113/jphysiol.2003.043414
Abstract
The aim of this study was to determine the effects of a clinically relevant single course of prenatal betamethasone in the rat on growth parameters with particular reference to brain cell proliferation and apoptosis. We report that administration of 170 μg kg−1 betamethasone twice within 4 h to E20 pregnant rats conveys moderate somatic growth retardation. Further, using a measure of brain cell proliferation independent of blood-brain barrier (BBB) permeability, we demonstrate for the first time that betamethasone is chronically anti-proliferative to brain cells without inducing caspase-3-mediated apoptosis. More importantly we show that there is a significant and sexually divergent rebound of neural proliferation which occurs earlier in males than in females and continues until at least 21 days of postnatal life. BBB permeability to [3H]thymidine was significantly increased by steroid treatment re-iterating the fact that tracer studies not correcting for BBB permeability, such as bromodeoxyuridine (BrdU), may be questionable in this type of study. Further, prenatal steroid treatment did not alter postnatal corticosterone levels. In summary we show that prenatal betamethasone conveys significant and long-lasting side effects and that its human clinical application in preterm labour needs more careful consideration as compared to the relative ease with which it is prescribed today.This publication has 61 references indexed in Scilit:
- Glucocorticoid exposure at the dose used clinically alters cytoskeletal proteins and presynaptic terminals in the fetal baboon brainJournal Of Physiology-London, 2003
- Glucocorticoid exposure at the dose used clinically alters cytoskeletal proteins and presynaptic terminals in the fetal baboon brainJournal Of Physiology-London, 2002
- Effect of betamethasone on maternal glucoseThe Journal of Maternal-Fetal & Neonatal Medicine, 2002
- Modulation of fibroblast growth factor-2 by stress and corticosteroids: from developmental events to adult brain plasticityBrain Research Reviews, 2001
- Head circumference in ELBW babies is associated with learning difficulties and cognition but not ADHD in the school-aged childDevelopmental Medicine and Child Neurology, 1999
- Head circumference in ELBW babies is associated with learning difficulties and cognition but not ADHD in the school‐aged childDevelopmental Medicine and Child Neurology, 1999
- Neonatal effects and serum cortisol levels after multiple courses of maternal corticosteroidsObstetrics & Gynecology, 1997
- Tracer dose and availability time of thymidine and bromodeoxyuridine: application of bromodeoxyuridine in cell kinetic studiesCell Proliferation, 1990
- The neonatal cerebellum: The highest level of glucocorticoid receptors in the brainDevelopmental Brain Research, 1984
- Radioimmunoassay of corticosterone, cortisol and cortisone: Their application to human cord and maternal plasmaJournal of Steroid Biochemistry, 1978