Control of Concentration Gradients of Pyruvate and Lactate Across Cell Membranes in Blood
- 1 September 1956
- journal article
- research article
- Published by American Physiological Society in Journal of Applied Physiology
- Vol. 9 (2), 163-170
- https://doi.org/10.1152/jappl.1956.9.2.163
Abstract
The relationships of extracellular and intracellular concentrations of pyruvate and lactate ions were investigated using fresh human blood cells. Oxygen, CO2 and ph were constant within narrow limits because arterial blood was used, collected at '6° to '10°C and denatured in less than 3 minutes. Plasma values were derivable from whole blood concentrations by multiplying by 1.21 and 1.07, respectively, for pyruvate and lactate (per liter of plasma or blood water). Plasma/cell ratios were 2.30 and 1.37. These factors were the same at all levels of blood pyruvate and lactate during rest and exercise, fever, hyperventilation and thiamine deficiency. The values of cell membrane gradients and their behavior during completely in vivo experiments suggested that lactate moved passively, and freely, across the membrane, while the pyruvate gradient resulted from metabolic activity of the cells plus passive diffusion through a membrane of relatively high resistance. Submitted on February 6, 1956Keywords
This publication has 6 references indexed in Scilit:
- Distribution of ions in suspensions of human erythrocytesThe Journal of Physiology, 1952
- ANALYSIS OF FACTORS CONCERNED IN REGULATION OF BREATHING IN EXERCISEPhysiological Reviews, 1950
- RENAL TUBULAR REABSORPTION, METABOLIC UTILIZATION AND ISOMERIC FRACTIONATION OF LACTIC ACID IN THE DOGAmerican Journal of Physiology-Legacy Content, 1946
- THE DISTRIBUTION OF LACTIC ACID IN HUMAN BLOODAmerican Journal of Physiology-Legacy Content, 1942
- THE ACID-BASE EQUILIBRIUM OF THE BLOOD IN EXERCISEAmerican Journal of Physiology-Legacy Content, 1942
- PATHOLOGICAL VARIATIONS IN BLOOD AND SPINAL FLUID PYRUVIC ACID 1JCI Insight, 1942