Herpes Simplex Virus Type-1 Glycoprotein D Gene: Nucleotide Sequence and Expression in Escherichia coli
- 22 October 1982
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 218 (4570), 381-384
- https://doi.org/10.1126/science.6289440
Abstract
The protein coding region of the herpes simplex virus type-1 glycoprotein D (gD) gene was mapped, and the nucleotide sequence was determined. The predicted amino acid sequence of the gD polypeptide was found to contain a number of features in common with other virus glycoproteins. Insertion of this protein coding region into a bacterial expressor plasmid enabled synthesis in Escherichia coli of an immunoreactive gD-related polypeptide. The potential of this system for preparation of a type-common herpes simplex virus vaccine is discussed.Keywords
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