Regulation of testicular tight junctions by gonadotrophins in the adult Djungarian hamster in vivo
Open Access
- 1 June 2008
- journal article
- Published by Bioscientifica in Reproduction
- Vol. 135 (6), 867-877
- https://doi.org/10.1530/rep-07-0572
Abstract
This study aimed to assess the effect of gonadotrophin suppression and FSH replacement on testicular tight junction dynamics and blood–testis barrier (BTB) organisationin vivo, utilising the seasonal breeding Djungarian hamster. Confocal immunohistology was used to assess the cellular organisation of tight junction proteins and real-time PCR to quantify tight junction mRNA. The effect of tight junction protein organisation on the BTB permeability was also investigated using a biotin-linked tracer. Tight junction protein (claudin-3, junctional adhesion molecule (JAM)-A and occludin) localisation was present but disorganised after gonadotrophin suppression, while mRNA levels (claudin-11, claudin-3 and occludin) were significantly (two- to threefold) increased. By contrast, both protein localisation and mRNA levels for the adaptor protein zona occludens-1 decreased after gonadotrophin suppression. FSH replacement induced a rapid reorganisation of tight junction protein localisation. The functionality of the BTB (as inferred by biotin tracer permeation) was found to be strongly associated with the organisation and localisation of claudin-11. Surprisingly, JAM-A was also recognised on spermatogonia, suggesting an additional novel role for this protein in trans-epithelial migration of germ cells across the BTB. It is concluded that gonadotrophin regulation of tight junction proteins forming the BTB occurs primarily at the level of protein organisation and not gene transcription in this species, and that immunolocalisation of the organised tight junction protein claudin-11 correlates with BTB functionality.Keywords
This publication has 58 references indexed in Scilit:
- JAM-A is present in mammalian spermatozoa where it is essential for normal motilityDevelopmental Biology, 2008
- A seamless trespass: germ cell migration across the seminiferous epithelium during spermatogenesisThe Journal of cell biology, 2007
- The conserved transcriptome in human and rodent male gametogenesisProceedings of the National Academy of Sciences of the United States of America, 2007
- Coxsackie and adenovirus receptor (CAR) is a product of Sertoli and germ cells in rat testes which is localized at the Sertoli–Sertoli and Sertoli–germ cell interfaceExperimental Cell Research, 2007
- C-type natriuretic peptide regulates blood–testis barrier dynamics in adult rat testesProceedings of the National Academy of Sciences of the United States of America, 2007
- The effect of testosterone, dihydrotestosterone and oestradiol on the re-initiation of spermatogenesis in the adult photoinhibited Djungarian hamsterJournal of Endocrinology, 2007
- Molecular Basis of Epithelial Barrier Regulation: From Basic Mechanisms to Clinical ApplicationThe American Journal of Pathology, 2006
- Adult Sertoli Cells Are Not Terminally Differentiated in the Djungarian Hamster: Effect of FSH on Proliferation and Junction Protein Organization1Biology of Reproduction, 2006
- Interaction of Junctional Adhesion Molecule with the Tight Junction Components ZO-1, Cingulin, and OccludinPublished by Elsevier BV ,2000
- Development of the blood‐testis barrier and changes in vascular permeability at puberty in ratsInternational Journal of Andrology, 1988