Tert-Butyl Hydroperoxide-Induced Injury In Isolated Rat Hepatocytes: A Model for Studying Anti-Hepatotoxic Crude Drugs

Abstract

Tert-butyl hydroperoxide induces in freshly isolated rat hepatocytes malonaldehyde formation and lacticodehydrogenase and aspartate aminotransferase leakage. This model, when adapted to crude extracts, permits the demonstration of both anti-lipoperoxidant and antihepatotoxic activity of reference products like quercetin and silymarin and plant extracts like Rosmarinus officinalis and Eschscholzia californica.