The role of lipid metabolism in the acquisition of desiccation tolerance in Craterostigma plantagineum: a comparative approach
- 7 June 2013
- journal article
- research article
- Published by Wiley in The Plant Journal
- Vol. 75 (5), 726-741
- https://doi.org/10.1111/tpj.12241
Abstract
Dehydration leads to different physiological and biochemical responses in plants. We analysed the lipid composition and the expression of genes involved in lipid biosynthesis in the desiccation-tolerant plant Craterostigma plantagineum. A comparative approach was carried out with Lindernia brevidens (desiccation tolerant) and two desiccation-sensitive species, Lindernia subracemosa and Arabidopsis thaliana. In C. plantagineum the total lipid content remained constant while the lipid composition underwent major changes during desiccation. The most prominent change was the removal of monogalactosyldiacylglycerol (MGDG) from the thylakoids. Analysis of molecular species composition revealed that around 50% of 36:x (number of carbons in the acyl chains: number of double bonds) MGDG was hydrolysed and diacylglycerol (DAG) used for phospholipid synthesis, while another MGDG fraction was converted into digalactosyldiacylglycerol via the DGD1/DGD2 pathway and subsequently into oligogalactolipids by SFR2. 36:x-DAG was also employed for the synthesis of triacylglycerol. Phosphatidic acid (PA) increased in C. plantagineum, L. brevidens, and L. subracemosa, in agreement with a role of PA as an intermediate of lipid turnover and of phospholipase D in signalling during desiccation. 34:x-DAG, presumably derived from de novo assembly, was converted into phosphatidylinositol (PI) in C. plantagineum and L. brevidens, but not in desiccation-sensitive plants, suggesting that PI is involved in acquisition of desiccation tolerance. The accumulation of oligogalactolipids and PI in the chloroplast and extraplastidial membranes, respectively, increases the concentration of hydroxyl groups and enhances the ratio of bilayer- to non-bilayer-forming lipids, thus contributing to protein and membrane stabilization.Keywords
This publication has 61 references indexed in Scilit:
- The lysine-rich motif of intrinsically disordered stress protein CDeT11-24 from Craterostigma plantagineum is responsible for phosphatidic acid binding and protection of enzymes from damaging effects caused by desiccationJournal of Experimental Botany, 2012
- DGAT1andPDAT1Acyltransferases Have Overlapping Functions inArabidopsisTriacylglycerol Biosynthesis and Are Essential for Normal Pollen and Seed DevelopmentPlant Cell, 2009
- The K-Segment of Maize DHN1 Mediates Binding to Anionic Phospholipid Vesicles and Concomitant Structural ChangesPlant Physiology, 2009
- Plant phospholipid signaling: "in a nutshell"Journal of Lipid Research, 2009
- An Inositolphosphorylceramide Synthase Is Involved in Regulation of Plant Programmed Cell Death Associated with Defense inArabidopsisPlant Cell, 2008
- Phospholipase Dα3 Is Involved in the Hyperosmotic Response in ArabidopsisPlant Cell, 2008
- Lindernia brevidens: a novel desiccation‐tolerant vascular plant, endemic to ancient tropical rainforestsThe Plant Journal, 2008
- Structure and Function of a Mitochondrial Late Embryogenesis Abundant Protein Are Revealed by DesiccationPlant Cell, 2007
- Enzymatic degradation of polar lipids in Vigna unguiculata leaves and influence of drought stressPhysiologia Plantarum, 1998
- Chemical structures of mono-, di-, tri- and tetraglycosyl glycerides in rice branBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979