Altered morphology and increased cell adhesiveness of Chinese hamster ovary cells cultured on fibrin
- 1 February 1977
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 90 (2), 351-359
- https://doi.org/10.1002/jcp.1040900220
Abstract
Chinese hamster ovary cells cultivated on fibrin exhibited different characteristics from cells growing on plastic. While sparsely plated cells on plastic dishes had an epithelioid morphology, cells on fibrin assumed a round shape and then converted to a stretched form with protruded processes that increased with cell density. Within a few days, cells fibrinolysed adjacent fibrin and returned to the morphology seen in plastic dishes. When fibrinolysis was inhibited by ϵ-aminocaproic acid (EACA), cells continued to grow on the fibrin for a longer period and showed dense, criss-crossed fibroblast-type congestion. Whereas, cells on plastic maintained pavement-like epithelioid appearance when they grew to a confluent monolayer. The other altered characteristics on fibrin was increased accumulation of cells in multilayers. Normally as Chinese hamster cells on plastic proliferate, many cells float into the medium instead of piling up after they form a monolayer. On the other hand, cells on fibrin, being maintained by the addition of EACA, remained adherent, piling up multilayers instead of floating into the medium. A possible explanation of these findings is that the surface properties of the stretched cells on fibrin are altered to make them more adhesive. A possible link of these characteristics of the cells on fibrin to tumor cell behavior in vivo is discussed.Keywords
This publication has 15 references indexed in Scilit:
- Increased protease levels in transformed cells: A casein overlay assay for the detection of plasminogen activator productionCell, 1974
- PROPERTIES OF PLASMINOGEN ACTIVATORS FORMED BY NEOPLASTIC HUMAN CELL CULTURESThe Journal of Experimental Medicine, 1974
- Purification and characterization of a cellular fibrinolytic factor associated with oncogenic transformation: The plasminogen activator from SV-40-transformed hamster cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- FIBRINOLYSIS ASSOCIATED WITH ONCOGENIC TRANSFORMATIONThe Journal of Experimental Medicine, 1973
- The effect of inhibitors of RNA and protein synthesis on dibutyryl cyclic AMP mediated morphological transformations of Chinese hamster ovary cells in vitroBiochemical and Biophysical Research Communications, 1973
- AN ENZYMATIC FUNCTION ASSOCIATED WITH TRANSFORMATION OF FIBROBLASTS BY ONCOGENIC VIRUSESThe Journal of Experimental Medicine, 1973
- AN ENZYMATIC FUNCTION ASSOCIATED WITH TRANSFORMATION OF FIBROBLASTS BY ONCOGENIC VIRUSESThe Journal of Experimental Medicine, 1973
- Further Changes in Differentiation State Accompanying the Conversion of Chinese Hamster Cells to Fibroblastic Form by Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate and HormonesProceedings of the National Academy of Sciences, 1971
- Morphological Transformation of Chinese Hamster Cells by Dibutyryl Adenosine Cyclic 3′:5′-Monophosphate and TestosteroneProceedings of the National Academy of Sciences of the United States of America, 1971
- Warfarin Treatment of Mice Bearing Autochthonous Tumors: Effect on Spontaneous MetastasesScience, 1968