LEUKOCYTE LOCOMOTION AND CHEMOTAXIS
Open Access
- 1 February 1973
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 137 (2), 387-410
- https://doi.org/10.1084/jem.137.2.387
Abstract
Polymorphonuclear leukocyte (PMN) locomotion and chemotaxis have been evaluated by direct microscopic observation of individual cells in thin slide-cover slip preparations, and also by observations on populations of cells migrating into a Millipore filter. The direct microscopic method used the polarity of the locomoting PMNs (broad, advancing lamellipodium and knoblike constriction at the rear) to record the direction of movement. The Boyden chamber Millipore assay was made more reliable by following the front of cells advancing into the filter, rather than counting the number of cells on the lower filter surface. Special modifications of the Millipore assay were necessary in order to distinguish between influences on rate of locomotion and true chemotaxis.Keywords
This publication has 16 references indexed in Scilit:
- THE RELATIONSHIP OF THE CHEMOTACTIC BEHAVIOR OF THE COMPLEMENT-DERIVED FACTORS, C3a, C5a, AND C567, AND A BACTERIAL CHEMOTACTIC FACTOR TO THEIR ABILITY TO ACTIVATE THE PROESTERASE 1 OF RABBIT POLYMORPHONUCLEAR LEUKOCYTESThe Journal of Experimental Medicine, 1972
- Re-assessment of Boyden's technique for measuring chemotaxisJournal of Immunological Methods, 1972
- Analysis of individual leucocyte behavior during chemotaxisExperimental Cell Research, 1972
- A PREALBUMIN ACTIVATOR OF PREKALLIKREINThe Journal of Experimental Medicine, 1972
- Eosinophil cell separation from human peripheral blood.1970
- Immunoglobulin G as Possible Precursor of Chemotactic FactorNature, 1970
- Studies on chemotaxis. XI. Effect on neutrophils of lysosomal and other subcellular fractions from leukocytes.1969
- THE ISOLATION AND SELECTED PROPERTIES OF BLOOD MONOCYTESThe Journal of Experimental Medicine, 1966
- THE CHEMOTACTIC EFFECT OF MIXTURES OF ANTIBODY AND ANTIGEN ON POLYMORPHONUCLEAR LEUCOCYTESThe Journal of Experimental Medicine, 1962
- PHAGOCYTIN: A BACTERICIDAL SUBSTANCE FROM POLYMORPHONUCLEAR LEUCOCYTESThe Journal of Experimental Medicine, 1956