The Murine cot Proto‐oncogene: Genome Structure and Tissue‐specific Expression
Open Access
- 1 May 1993
- journal article
- research article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 84 (5), 518-525
- https://doi.org/10.1111/j.1349-7006.1993.tb00170.x
Abstract
We cloned and analyzed the murine cot proto‐oncogene and examined its tissue‐specific expression in fetal, newborn and adult mice. Genomic cot DNA consists of eight exons, spanning more than 25 kb, and all intron‐exon borders are well conserved as compared to the human homolog. Analysis of the full‐length cot cDNA revealed that it contained an open reading frame of 1,401 nucleotides, like human cot proto‐oncogene. The sequence identity between murine and human cot gene is 84.4% at the nucleotide level and 93.9% at the deduced amino acid level. On northern blot analysis of poly (A)+ RNA, the cot message was detected at 2.9 kb in size. Expression of the cot gene was observed in many tissues from fetal to adult mice, though the level of expression was low in all tissues examined.Keywords
This publication has 21 references indexed in Scilit:
- A Retroviral Oncogene, akt , Encoding a Serine-Threonine Kinase Containing an SH2-Like RegionScience, 1991
- [2] Protein kinase catalytic domain sequence database: Identification of conserved features of primary structure and classification of family membersMethods in Enzymology, 1991
- cDNA cloning and sequence determination of rat membrane-associated phospholipase A2Biochemical and Biophysical Research Communications, 1989
- One-sided polymerase chain reaction: the amplification of cDNA.Proceedings of the National Academy of Sciences of the United States of America, 1989
- Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytesNature, 1988
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987
- Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform ExtractionAnalytical Biochemistry, 1987
- The primary structure of the putative oncogene pim-1 shows extensive homology with protein kinasesCell, 1986
- A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradationCell, 1986
- The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 geneNucleic Acids Research, 1986