Immunization of Chickens with VP2 Protein of Infectious Bursal Disease Virus Expressed in Arabidopsis thaliana
- 1 September 2004
- journal article
- research article
- Published by American Association of Avian Pathologists (AAAP) in Avian Diseases
- Vol. 48 (3), 663-668
- https://doi.org/10.1637/7074
Abstract
Transgenic plants represent a safe, effective, and inexpensive way to produce vaccines. The immunogenicity of VP2 protein of an infectious bursal disease (IBD) virus variant E isolate expressed in transgenic Arabidopsis thaliana was compared with a commercial vaccine in specific-pathogen-free broiler chickens. The VP2 coding sequence was isolated and integrated into A. thaliana genome by Agrobacterium tumefaciens–mediated transformation. Soluble VP2 expressed in transgenic plants was used to immunize chickens. Chickens receiving oral immunization with plant-derived VP2 at 1 and 3 wk of age had an antibody response using enzyme-linked immunosorbent assay and 80% protection against challenge infection at 4 wk. Chickens primed with a commercial vaccine at 1 wk followed by an oral booster with VP2 expressed in plants at 3 wk of age showed 90% protection. Chickens immunized with a commercial vaccine at 1 and 3 wk showed 78% protection. Results supported the efficacy of plant-produced VP2 as a vaccine against IBD. Nota de Investigación—Immunización de pollos con la proteína VP2 del virus de la enfermedad infecciosa de la bolsa expresada en Arabidopsis thaliana. Las plantas transgénicas representan una manera segura, efectiva y económica de producir vacunas. La inmunogenicidad de la proteína VP2 expresada en Arabidopsis thaliana de un aislamiento de la variante E del virus fue comparada con una vacuna comercial en aves libres de patógenos específicos. La secuencia codificadora de la proteína VP2 se aisló e integró al genoma de A. thaliana por medio de la transformación mediada por Agrobacterium tumefaciens. La VP2 soluble expresada en plantas transgénicas fue utilizada para inmunizar pollos. Los pollos que recibieron a la primera y tercera semanas de edad la inmunización oral con VP2 derivada de plantas, mostraron una respuesta de anticuerpos detectada por el ensayo de inmunoabsorción con enzimas ligadas, además de una protección de un 80% contra el desafio a las cuatro semanas. Los pollos primovacunados con una vacuna comercial a la semana de edad seguido de un refuerzo a las tres semanas con VP2 expresada en plantas, mostraron 90% de protección. Los pollos inmunizados con una vacuna comercial a la primera y tercera semana mostraron 78% de protección. Los resultados confirmaron la eficacia de la proteína VP2 producida en plantas como vacuna contra el virus de la enfermedad infecciosa. Abbreviations: B2 = Bursine 2; ELISA = enzyme-linked immunosorbant assay; IBD = infectious bursal disease; IBDV = infectious bursal disease virus; mAb = monoclonal antibody; N = untransformed; PBS = phosphate-buffered saline; RT-PCR = reverse transcription–polymerase chain reaction; SDS-PAGE = sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SPF = specific-pathogen-free; SQ = subcutaneously; T = V-3 plantKeywords
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