Peptide with Angiotensin I-Converting Enzyme Inhibitory Activity from Hydrolyzed Corn Gluten Meal
- 24 August 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 55 (19), 7891-7895
- https://doi.org/10.1021/jf0705670
Abstract
Corn gluten meal (CGM) was hydrolyzed by Alcalase after starch removal of CGM was applied as a pretreatment. A new inhibitory peptide for angiotensin I-converting enzyme (ACE) was isolated from the hydrolysate of CGM with the use of Bio-Rad P-2 gel filtration and followed by reverse-phase high-performance liquid chromatography (RP-HPLC). The sequence of the active peptide was determined to be Ala-Tyr after the application of amino acid analysis and HPLC/MS. The IC50 of the peptide was 14.2 microM, and it was not affected by preincubation with 30 mU of ACE at 37 degrees C for 3 h. Ala-Tyr also exerted antihypertensive effects after oral administration to spontaneously hypertensive rats. A maximal reduction of systolic blood pressure of 9.5 mmHg was observed 2 h after oral administration of Ala-Tyr at doses of 50 mg/kg.Keywords
This publication has 21 references indexed in Scilit:
- Preparation of corn gluten hydrolysate with angiotensin I converting enzyme inhibitory activity and its solubility and moisture sorptionProcess Biochemistry, 2004
- Isolation and Antihypertensive Effect of Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from Spinach RubiscoJournal of Agricultural and Food Chemistry, 2003
- Angiotensin‐I‐converting enzyme inhibitory peptides from tryptic hydrolysate of bovine αS2‐caseinFEBS Letters, 2002
- Latent production of angiotensin I‐converting enzyme inhibitors from buckwheat proteinJournal of Peptide Science, 2002
- Classification and Antihypertensive Activity of Angiotensin I‐Converting Enzyme Inhibitory Peptides Derived from Food ProteinsJournal of Food Science, 2000
- Critical appraisal of the JNC VI, WHO/ISH and BHS guidelines for essential hypertensionExpert Opinion on Pharmacotherapy, 2000
- Depressor Effect of Wheat Germ Hydrolysate and Its Novel Angiotensin I-Converting Enzyme Inhibitory Peptide, Ile-Val-Tyr, and the Metabolism in Rat and Human Plasma.Biological & Pharmaceutical Bulletin, 2000
- Blood pressure reduction and vasodilatory effects in vivo of peptides originating from sardine muscle.Nippon Eiyo Shokuryo Gakkaishi, 1989
- Clinical experience with blockade of the renin-angiotensin-aldosterone system by an oral converting-enzyme inhibitor (SQ 14,225, captopril) in hypertensive patientsProgress in Cardiovascular Diseases, 1978
- Design of Specific Inhibitors of Angiotensin-Converting Enzyme: New Class of Orally Active Antihypertensive AgentsScience, 1977