The Effect of Adrenalectomy on Leptin Levels and Some Metabolic Parameters in Rats with Diet-Induced Obesity.
- 1 January 2002
- journal article
- Published by Pharmaceutical Society of Japan in Biological & Pharmaceutical Bulletin
- Vol. 25 (5), 580-583
- https://doi.org/10.1248/bpb.25.580
Abstract
Recently, there has been many investigations on the relationship between leptin and obesity, which is the main health problem in developed countries. In some reports, it has been claimed that the adrenalectomy has lead to weight loss and thus prevented obesity induced in rodents in various ways. It has also been accepted that diet-induced obesity in animals is very similar to obesity in humans beings. In this study, obesity has been developed with high-calorie diet given for 8 weeks in Sprague-Dawley rats. Then, it has been investigated how leptin and some metabolic parameters change in blood samples obtained from rats 15 d after adrenalectomy. Leptin levels was determined with Radio Immun Assay (RIA, Linco Research Co) method. Our study showed that, there were statistically significant increases in leptin (p<0.001), glucose (p<0.05), triglyceride (p<0.01) levels in diet-induced obese rats (n=19) when compared with the findings of control rats, lean ones (n=16), (Tables 3, 4). Adrenalectomy led to decreased serum leptin (p<0.001) and triglyceride (p<0.01) levels both in the obese and lean rats (Table 5). As a conclusion, it could be claimed that the decrease in leptin levels may be attributed to reduced adipose tissue due to adrenalectomy. On the other hand, the decreases in glucose and triglyceride levels might be the consequence of reduced lipogenesis and impaired gluconeogenesis with the effect of adrenalectomy. It was concluded that adrenalectomy might prevent obesity by affecting leptin and intermediate metabolism.Keywords
This publication has 14 references indexed in Scilit:
- Negative regulation of leptin by chronic high-glycemic index starch dietMetabolism, 2000
- Inhibition of Food Response to Intracerebroventricular Injection of Leptin Is Attenuated in Rats With Diet-Induced ObesityDiabetes, 1997
- Acute and prolonged administration of glucocorticoids (methylprednisolone) does not affect plasma leptin concentration in humansInternational Journal of Obesity, 1997
- Adrenalectomy Prevents the Obesity Syndrome Produced by Chronic Central Neuropeptide Y Infusion in Normal RatsDiabetes, 1997
- Diet-induced obese mice develop peripheral, but not central, resistance to leptin.JCI Insight, 1997
- Leptin: The Tale of an Obesity GeneDiabetes, 1996
- Regulation of energy balance by leptinExperimental and Clinical Endocrinology & Diabetes, 1996
- Induction of ob Gene Expression by Corticosteroids Is Accompanied by Body Weight Loss and Reduced Food IntakeJournal of Biological Chemistry, 1995
- Positional cloning of the mouse obese gene and its human homologueNature, 1994
- Hypothalamic-pituitary-adrenal axis function in the Zucker obese ratEndocrinology, 1992