Analysis of cell variants showing differential susceptibilities to radiation‐ or chemical‐induced neoplastic transformation: Differences in their responses to growth factors
- 1 April 1989
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 139 (1), 18-23
- https://doi.org/10.1002/jcp.1041390104
Abstract
The induction of DNA synthesis in quiescent, density-arrested Balb/c 3T3 cells is known to be controlled by the sequential action of at least two functionally distinct sets of growth factors, so-called “competence factors” and “progression factors.” Here we examined this induction pathway in Balb/c 3T3 A31-l variants, which showed differential susceptibilities to radiation- and chemical-induced neoplastic transformation despite their similar susceptibilities to radiation- or chemical-induced cell killing and mutagenesis. DNA synthesis was acquired only with the exposure to progression factors in a highly susceptible cell variant (A31-l-13) whereas both competence factors and progression factors were required for a less susceptible cell variant (A31-l-1). The competent state constitutively produced by an autologous mechanism in the highly transformation-susceptible A31-l-13 cells suggests the existence of an endogenous promoter that acts for the expression of the transformed phenotype in an autocrine fashion when the cells have been initiated by radiation or chemical carcinogens. The growth factor requirements acting as a determining factor for susceptibilities to transformation are discussed.Keywords
This publication has 18 references indexed in Scilit:
- Induction by growth factors from platelets of the focus-forming transformed phenotype in carcinogen-treated C3H /10T1 /2 fibroblastsCarcinogenesis: Integrative Cancer Research, 1988
- Dissociation of cellular transformation from platelet‐derived growth factor independenceJournal of Cellular Physiology, 1986
- Neuromedin B: A novel bombesin-like peptide identified in porcine spinal cordBiochemical and Biophysical Research Communications, 1983
- A New Tumor-Promoting Agent, Dihydroteleocidin B, Markedly Enhances Chemically Induced Malignant Cell TransformationScience, 1982
- Cell Variants Showing Differential Susceptibility to Ultraviolet Light—Induced TransformationScience, 1980
- The tumor promoter 12‐0‐tetradecanoyl‐phorbol‐13‐acetate enhances the proliferative response of Balb/c‐3T3 cells to hormonal growth factorsJournal of Cellular Physiology, 1979
- Characterization of a gastrin releasing peptide from porcine non-antral gastric tissueBiochemical and Biophysical Research Communications, 1979
- Control of the Balb/c‐3T3 cell cycle by nutrients and serum factors: Analysis using platelet‐derived growth factor and platelet‐poor plasmaJournal of Cellular Physiology, 1979
- Development of 3T3‐like lines from Balb/c mouse embryo cultures: Transformation susceptibility to SV40Journal of Cellular Physiology, 1968