Adaptive optical fluorescence microscopy
Top Cited Papers
- 1 April 2017
- journal article
- review article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 14 (4), 374-380
- https://doi.org/10.1038/nmeth.4218
Abstract
The past quarter century has witnessed rapid developments of fluorescence microscopy techniques that enable structural and functional imaging of biological specimens at unprecedented depth and resolution. The performance of these methods in multicellular organisms, however, is degraded by sample-induced optical aberrations. Here I review recent work on incorporating adaptive optics, a technology originally applied in astronomical telescopes to combat atmospheric aberrations, to improve image quality of fluorescence microscopy for biological imaging.Keywords
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