Mechanical Loading of Stem Cells for Improvement of Transplantation Outcome in a Model of Acute Myocardial Infarction: The Role of Loading History
- 1 June 2012
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering, Part A
- Vol. 18 (11-12), 1101-1108
- https://doi.org/10.1089/ten.tea.2011.0285
Abstract
Stem cell therapy for tissue repair is a rapidly evolving field and the factors that dictate the physiological responsiveness of stem cells remain under intense investigation. In this study we hypothesized that the mechanical loading history of muscle-derived stem cells (MDSCs) would significantly impact MDSC survival, host tissue angiogenesis, and myocardial function after MDSC transplantation into acutely infarcted myocardium. Mice with acute myocardial infarction by permanent left coronary artery ligation were injected with either nonstimulated (NS) or mechanically stimulated (MS) MDSCs. Mechanical stimulation consisted of stretching the cells with equibiaxial stretch with a magnitude of 10% and frequency of 0.5 Hz. MS cell-transplanted hearts showed improved cardiac contractility, increased numbers of host CD31+ cells, and decreased fibrosis, in the peri-infarct region, compared to the hearts treated with NS MDSCs. MS MDSCs displayed higher vascular endothelial growth factor expression than NS cells in vitro. These findings highlight an important role for cyclic mechanical loading preconditioning of donor MDSCs in optimizing MDSC transplantation for myocardial repair.This publication has 48 references indexed in Scilit:
- Cellular Antioxidant Levels Influence Muscle Stem Cell TherapyMolecular Therapy, 2010
- Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in CultureScience, 2010
- Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate techniqueNature Protocols, 2008
- Muscle-derived stem cells isolated as non-adherent population give rise to cardiac, skeletal muscle and neural lineagesExperimental Cell Research, 2008
- HIF-1α induced-VEGF overexpression in bone marrow stem cells protects cardiomyocytes against ischemiaJournal of Molecular and Cellular Cardiology, 2007
- Long-Term Self-Renewal of Postnatal Muscle-derived Stem CellsMolecular Biology of the Cell, 2005
- Regeneration of dystrophin-expressing myocytes in the mdx heart by skeletal muscle stem cellsGene Therapy, 2005
- Cell therapy for cardiovascular disease: what cells, what diseases and for whom?Nature Clinical Practice Cardiovascular Medicine, 2005
- Dynamics and mediators of acute graft attrition after myoblast transplantation to the heartThe FASEB Journal, 2004
- Identification of a novel population of muscle stem cells in miceThe Journal of cell biology, 2002