Targeted expression of Cre recombinase to adipose tissue of transgenic mice directs adipose-specific excision of loxP-flanked gene segments
Open Access
- 1 June 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (12), 2543-2545
- https://doi.org/10.1093/nar/25.12.2543
Abstract
Functional analysis of mammalian genes relies, in part, on targeted mutations generated by homologous recombination in mice. We have developed a strategy for adipose-specific inactivation of loxP-floxed gene segments. Transgenic mice have been established that express Cre recombinase under the control of the adipose-specific aP2 enhancer/promoter. Crossing of the aP2/Cre mice with any loxP-floxed gene will facilitate its functional analysis in adipose tissue.Keywords
This publication has 14 references indexed in Scilit:
- Subregion- and Cell Type–Restricted Gene Knockout in Mouse BrainCell, 1996
- Targeted expression of a toxin gene to adipose tissue: transgenic mice resistant to obesity.Genes & Development, 1993
- Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targetingCell, 1993
- Tissue- and site-specific DNA recombination in transgenic mice.Proceedings of the National Academy of Sciences, 1992
- Targeted oncogene activation by site-specific recombination in transgenic mice.Proceedings of the National Academy of Sciences, 1992
- A fat-specific enhancer is the primary determinant of gene expression for adipocyte P2 in vivo.Proceedings of the National Academy of Sciences of the United States of America, 1990
- Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase.1990
- Altering the Genome by Homologous RecombinationScience, 1989
- Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1.Proceedings of the National Academy of Sciences, 1988
- Bacteriophage P1 site-specific recombinationJournal of Molecular Biology, 1981