Fate of germinated Bacillus anthracis spores in primary murine macrophages
Open Access
- 1 November 2001
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 42 (4), 931-938
- https://doi.org/10.1046/j.1365-2958.2001.02695.x
Abstract
We investigated the fate of germinated Bacillus anthracis spores after their germination in Swiss murine peritoneal macrophages and in the cell line RAW264.7. We found that the lethal toxin and the oedema toxin are germ-associated factors that are essential for the survival of the vegetative form in host cells. We also found that pX02 is not involved in this complex pathogenic process. By transmission electron microscopy, we showed the tight interaction between the exosporium of the spore and the phagosomal membrane of the macrophage. Our data strongly suggest that the B. anthracis toxinogenic, unencapsulated Sterne strain (7702) does not multiply within macrophages. These results contributed to reveal the strategies used by B. anthracis to survive within the host and to reach the external medium where they proliferate.Keywords
This publication has 38 references indexed in Scilit:
- Role of Toxin Functional Domains in Anthrax PathogenesisInfection and Immunity, 2000
- Anthrax lethal factor cleaves MKK3 in macrophages and inhibits the LPS/IFNγ‐induced release of NO and TNFαFEBS Letters, 1999
- Germination of Bacillus anthracis spores within alveolar macrophagesMolecular Microbiology, 1999
- Anthrax Lethal Factor Cleaves the N-Terminus of MAPKKs and Induces Tyrosine/Threonine Phosphorylation of MAPKs in Cultured MacrophagesBiochemical and Biophysical Research Communications, 1998
- Anthrax lethal toxin-induced mitogenic response of human T-cellsFEMS Microbiology Letters, 1997
- Recent Advances in the Understanding of Resistance and Dormancy in Bacterial SporesJournal of Applied Bacteriology, 1977
- Cytological Features of Anthrax Spores Phagocytized in Vitro by Rabbit Alveolar MacrophagesThe Journal of Infectious Diseases, 1966
- Pathophysiology of AnthraxThe Journal of Infectious Diseases, 1966
- Purification of Factor I and Recognition of a Third Factor of the Anthrax ToxinMicrobiology, 1961
- The pathogenesis of anthrax following the administration of spores by the respiratory routeThe Journal of Pathology and Bacteriology, 1957