Electron donor effect on nitrate reduction pathway and kinetics in a mixed methanogenic culture
- 9 May 2007
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 98 (4), 756-763
- https://doi.org/10.1002/bit.21487
Abstract
The effect of different electron donors on the pathway and kinetics of nitrate reduction in a sulfide-acclimated mixed, mesophilic (35°C) methanogenic culture was investigated. A mixture of dextrin and peptone, glucose, propionate, acetate, and H2/CO2 were used as substrates at an initial chemical oxygen demand of 1,500 mg/L and the initial nitrate concentration ranged between 0 and 300 mg N/L. The fastest nitrate reduction was observed in the H2/CO2 and acetate-fed cultures. In the case of propionate, nitrate reduction was the slowest followed by partial recovery of methanogenesis and accumulation of volatile fatty acids due to inhibition as a result of accumulation of denitrification intermediates. Similarly, accumulation of nitrite and nitric oxide and partial or complete inhibition of methanogenesis was observed in the H2/CO2-fed cultures. Methanogenesis completely recovered in the dextrin/peptone-, glucose-, and acetate-fed cultures at all nitrate levels. Denitrification was the dominant pathway of nitrate reduction in the propionate-, acetate-, and H2/CO2-fed cultures regardless of the COD/N value. However, both denitrification and dissimilatory nitrate reduction to ammonia (DNRA) were observed in the dextrin/peptone- and glucose-fed cultures and the degree of predominance of either of the two pathways was a function of the COD/N value. Therefore, the type of electron donor used affected both the nitrate reduction pathway and kinetics, as well as the recovery of fermentation and/or methanogenesis in the mixed methanogenic culture. Biotechnol. Biotechnol. Bioeng. 2007;98: 756–763.Keywords
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