Two functionally different regions in Fos are required for the sequence-specific DNA interaction of the Fos/Jun protein complex
- 1 April 1989
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 338 (6216), 589-590
- https://doi.org/10.1038/338589a0
Abstract
THE products of the cellular and retroviral fos genes associate with other nuclear proteins, among them the transcription factor AP1/Jun (see ref. 3 for a review). The Fos/Jun complex binds to a specific symmetrical DNA recognition sequence (termed TRE), thus stimulating transcription of the respective gene. Here, we show that two distinct regions in Fos are required for the formation of a Fos/Jun/TRE complex. These are the leucine zipper, involved in the association with Jun, and a directly adjacent basic region. Specific amino-acid substitutions in this basic, presumably alpha-helical, region abolish the interaction of Fos/Jun with the TRE but not the association of the two proteins. The functionally crucial amino acids are located in a region of Fos which is structurally similar to the putative DNA-binding sites in Jun and in the yeast transcriptional activator GCN4 (refs 15 and 16).Keywords
This publication has 23 references indexed in Scilit:
- Fos and jun: The AP-1 connectionCell, 1988
- The c-fos protein interacts with c-JunAP-1 to stimulate transcription of AP-1 responsive genesCell, 1988
- Transcriptional activation of c-jun during the G0/G1 transition in mouse fibroblastsNature, 1988
- Requirement for fos gene expression in the transcriptional activation of collagenase by other oncogenes and phorbol estersCell, 1988
- The Leucine Zipper: A Hypothetical Structure Common to a New Class of DNA Binding ProteinsScience, 1988
- Nucleoprotein complexes that regulate gene expression in adipocyte differentiation: direct participation of c-fosCell, 1987
- Purified transcription factor AP-1 interacts with TPA-inducible enhancer elementsCell, 1987
- Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factorCell, 1987
- Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteinsJournal of Molecular Biology, 1978
- Prediction of protein conformationBiochemistry, 1974