Inhibitory activity of Indian spice plant Cinnamomum zeylanicum extracts against Alternaria solani and Curvularia lunata, the pathogenic dematiaceous moulds
Open Access
- 7 March 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Annals of Clinical Microbiology and Antimicrobials
- Vol. 8 (1), 9
- https://doi.org/10.1186/1476-0711-8-9
Abstract
Dematiaceous moulds are pathogenic microorganisms and act as etiological agents of mycoses with different degrees of severity in humans and animals. These moulds also cause loss of food crops and storage food products. The information regarding antimicrobial efficacy of the plant preparations on these moulds is scanty. The present study reveals phytochemical characterization and the effect of bark and leaf extracts of Indian spice plant, Cinnamomum zeylanicum (Cz), against the growth of two species of dematiaceous moulds, Alternaria solani and Curvularia lunata. Cz bark and leaf samples were sequentially extracted in different solvents using Soxhlet apparatus. Phytochemical analyses of extracts were done as per standard protocols. The antifungal bioassay of extracts was done by hanging drop technique. The inhibition of fungal spore germination was monitored under influence of three different concentrations of extracts. The lowest test concentration (50 μg/ml) of extracts of Cz bark prepared into acetone and that of Cz leaf into petroleum ether and ethanol exhibited complete inhibition (100%) of spore germination in both the moulds. At 100 μg/ml concentration all the extracts showed about 50 to 100% inhibition. However, the treatment of the spores of the two fungal species with highest concentration (500 μg/ml) of bark and leaf extracts in all the solvents showed 100% fungicidal activity as it completely arrested the germination of spores. Relatively lower activity of aqueous extracts at 50 and 100 μg/ml concentrations suggests that the antifungal ingredients present in Cz bark and leaf are more soluble in organic solvents than water. The results demonstrated that the Cz bark and leaves contain certain fungicidal constituents exhibiting potential antimould activity against A. solani and C. lunata.Keywords
This publication has 21 references indexed in Scilit:
- Climatology of Dematiaceous Fungal KeratitisAmerican Journal of Ophthalmology, 2005
- Antimicrobial Activities and Phytochemical Profiles of Endemic Medicinal Plants of MauritiusPharmaceutical Biology, 2005
- Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia region of TurkeyFood Control, 2004
- Essential oils: their antibacterial properties and potential applications in foods—a reviewInternational Journal of Food Microbiology, 2004
- Identifying filamentous fungi: a clinical laboratory handbookRevista do Instituto de Medicina Tropical de São Paulo, 2003
- Effect of temperature, water activity, pH and some antimicrobials on the growth of Penicillium olsonii isolated from the surface of Spanish fermented meat sausageFood Microbiology, 2002
- Medicinal herbs in the United States: research needs.Environmental Health Perspectives, 1999
- Biological activities of some Turkish medicinal plantsPure and Applied Chemistry, 1998
- The normal mycoflora of commodities from Thailand. 2. Beans, rice, small grains and other commoditiesInternational Journal of Food Microbiology, 1994
- Fungi Associated with Bovine Abortion in the Northern Plains States (USA)Journal of Veterinary Diagnostic Investigation, 1992