Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle
Open Access
- 9 May 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 3 (1), srep01813
- https://doi.org/10.1038/srep01813
Abstract
Intracellular protein trafficking pathways are hijacked by viruses at various stages of viral life-cycle. Heterotetrameric adaptor protein complexes (APs) mediate vesicular trafficking at distinct intracellular sites and are essential for maintaining the organellar homeostasis. In the present study, we studied the effect of AP-1 and AP-3 deficiency on flavivirus infection in cells functionally lacking these proteins. We show that AP-1 and AP-3 participate in flavivirus life-cycle at distinct stages. AP-3-deficient cells showed delay in initiation of Japanese encephalitis virus and dengue virus RNA replication, which resulted in reduction of infectious virus production. AP-3 was found to colocalize with RNA replication compartments in infected wild-type cells. AP-1 deficiency affected later stages of dengue virus infection where increased intracellular accumulation of infectious virus was observed. Therefore, our results propose a novel role for AP-1 and AP-3 at distinct stages of infection of some of the RNA viruses.Keywords
This publication has 29 references indexed in Scilit:
- The Fifth Adaptor Protein ComplexPLoS Biology, 2011
- Mosquito-borne flaviviruses: overview of viral life-cycle and host-virus interactionsFuture Virology, 2011
- Flavivirus Cell Entry and Membrane FusionViruses, 2011
- Endosome–lysosome fusionBiochemical Society Transactions, 2010
- Efficient Assembly and Secretion of Recombinant Subviral Particles of the Four Dengue Serotypes Using Native prM and E ProteinsPLOS ONE, 2009
- AP-3-dependent trafficking and disease: the first decadeCurrent Opinion in Cell Biology, 2009
- Sorting of lysosomal proteinsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2008
- RNA interference screen for human genes associated with West Nile virus infectionNature, 2008
- AP‐1A and AP‐3A Lysosomal Sorting FunctionsTraffic, 2002
- μ1A-adaptin-deficient mice: lethality, loss of AP-1 binding and rerouting of mannose 6-phosphate receptorsThe EMBO Journal, 2000