Amplification of the metallothionein-I gene in cadmium-resistant mouse cells.
- 1 April 1981
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 78 (4), 2110-2114
- https://doi.org/10.1073/pnas.78.4.2110
Abstract
Friend leukemia cells resistant to cadmium toxicity were selected. More than 70% of total cysteine incorporation in these cells was into the metal-binding protein, metallothionein. We used cDNA and genomic DNA clones containing the metallothionein-I gene to measure the concentration of its mRNA, the rate of gene transcription, and the number of genes. On a per cell basis, optimally induced, cadmium-resistant cells have a 14-fold more metallothionein-I mRNA, a 6-fold higher rate of metallothionein-I gene transcription, and 6-fold more metallothionein-I genes than do nonresistant cells. Metaphase spreads revealed that the resistant cells are nearly tetraploid and contain, on the average, three very small chromosomes that are absent from non-resistant Friend cells.This publication has 17 references indexed in Scilit:
- Isolation and characterization of the mouse metallothionein-I gene.Proceedings of the National Academy of Sciences of the United States of America, 1980
- Integration and organization of Ti plasmid sequences in crown gall tumorsCell, 1980
- Conversion of bacteriophage fd into an efficient single-stranded DNA vector systemMolecular Genetics and Genomics, 1980
- A cadmium-resistant variant of the Chinese hamster (CHO) cell with increased metallothionein induction capacityExperimental Cell Research, 1979
- Amplified dihydrofolate reductase genes in unstably methotrexate-resistant cells are associated with double minute chromosomes.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Specific measurement of DNA in nuclei and nucleic acids using diaminobenzoic acidAnalytical Biochemistry, 1978
- The induction of ovalbumin and conalbumin mRNA by estrogen and progesterone in chick oviduct explant culturesCell, 1978
- Metallothionein accumulation in response to cadmium in a clonal rat liver cell line*1Toxicology and Applied Pharmacology, 1978
- Cadmium resistance and content of cadmium-binding protein in two enzyme-deficient mutants of mouse fibroblasts (L-cells)Biochemical Pharmacology, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970